Kallikrein 10 antibody (C-Term)

Catalog No. ABIN3043037

The Rabbit Polyclonal anti-Kallikrein 10 antibody has been validated for WB. It is suitable to detect Kallikrein 10 in samples from Human and Rat.

Quick Overview for Kallikrein 10 antibody (C-Term) (ABIN3043037)

Target: Kallikrein 10 (KLK10)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Kallikrein 10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Kallikrein 10 Antibody

Binding Specificity: AA 252-265, C-Term

Purpose: Anti-Kallikrein 10/KLK10 Antibody Picoband®

Sequence: SAQHPAVYTQ ICKY

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Kallikrein 10/KLK10 Antibody (ABIN3043037). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Kallikrein 10.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Elliott, M. B., Irwin, D. M., Diamandis, E. P. In silico identification and Bayesian phylogenetic analysis of multiple new mammalian kallikrein gene families. Genomics 88: 591-599, 2006. 2. Gan, L., Lee, I., Smith, R., Argonza-Barrett, R., Lei, H., McCuaig, J., Moss, P., Paeper, B., Wang, K. Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region. Gene 257: 119-130, 2000. 3. Polikoff, D., Kuo, W.-L., Cochran, J. F., Wernick, M., Kowbel, D., Myambo, K., Collins, C. C. Assignment of protease, serine-like 1 (PRSSL1) to human chromosome 19q13 by in situ hybridization and radiation hybrid mapping. Cytogenet. Cell Genet. 79: 147-148, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Kallikrein 10

Target: Kallikrein 10 (KLK10)

Alternative Name: KLK10

Background:

Synonyms: Kallikrein-10;3.4.21.-;Normal epithelial cell-specific 1;Protease serine-like 1;KLK10;NES1, PRSSL1;

Tissue Specificity: Expressed in breast, ovary and prostate.

Background: KLK10 (kallikrein-related peptidase 10) also known as kallikrein 10, PRSSL1 or NES1, belongs to the kallikrein subfamily of serine proteases, which are involved in a variety of enzymatic processes. Its encoded protein is secreted and may play a role in suppression of tumorigenesis in breast and prostate cancers.KLK10 also contains 12 conserved cysteines in its protease domain that are expected to form 6 disulfide bonds, as in other serine proteases. RT-PCR detected variable levels of KLK10 expression in nearly all of the 35 adult and fetal tissues examined. The KLK10 gene contains 6 exons, the first of which is noncoding. By fluorescence in situ hybridization, Polikoff et al. (1997) mapped the PRSSL1 gene to 19q13.3. By genomic sequence analysis, Gan et al. (2000) mapped the KLK10 gene within the KLK gene cluster on chromosome 19q13. This cluster includes 13 KLK genes and 5 pseudogenes.

Sequence Similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

Molecular Weight: 37 kDa

UniProt: O43240

Pathways: Complement System